It may affect lymphocyte activation in vitro, the distribution of leukocyte subpopulations, and the cell extraction performance.  Immunologists have advised us not to performed the IMMUNOBIOGRAM® during the first months of immunosuppressive therapy (during the induction therapy), as the treatment can reduce in a significant way the number and activity of T lymphocytes and the results could be seriously disrupted.  The advice is to perform it before kidney transplantation and wait at least 6 months after the transplantation to perform it again. Our studies, however, have focused until now in the maintenance phase of immunosuppressive treatment and all patients tested had been transplanted more than one year before.